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Abstract

STUDIES ON ACUTE TOXICITY PROFILE AND UTERINE MORPHOMETRIC ANALYSIS OF A SINGLE INTRA-UTERINE INJECTION OF RISUG, A NANOTECHNOLOGY BASED BIOMEDICINE IN RATS

Falgun Wanganuji Bansode, Anil Kumar Meena and Rama Kant Singh*

ABSTRACT

The non-hormonal contraceptive, named RISUG (an acronym for Reversible Inhibition of Sperm under Guidance) has expected to provide a valuable addition to the current options of male contraception. A recent study on intra-uterine infusion of RISUG has shown antifertility effects. Present study was conducted to assess the toxic effects of the RISUG in female Charles Foster rats. Healthy adult female rats of Charles Foster strain were randomly divided into two groups (Gr.) consisting 10 animals each. Gr. I. rats were injected with vehicle only (DMSO) in both uterine horns served as Control. Rats in G.II were injected with RISUG in the uterus bilaterally and observed for a period of 14 days. The initial (on day 0) and final (on day 15) body weights, food and water consumption in control and treated animals were recorded. Blood samples were collected in EDTA-coated vials for haematological and biochemical analysis. On day 15, autopsy of animals was done and body organs (viz. brain, heart, liver, lungs, kidney, adrenal, spleen, uterus and ovaries) were dissected out, weighed and fixed in formalin for histopathological study. Results showed that the body weights, absolute/relative organ weights, food and water consumption, hematological parameters, viz. Hgb(g%), RBC(x106/mm3), Hct(%), MCV(micron3), MCHC(g%), TLC(x103/mm3), DLC(%) of Polymorphs, Lymphocytes, Macrophages, Eosinophils and Platelets (x103/mm3), and biochemical analysis of marker enzymes, did not show any significant changes in RISUG-treated as compared to control rats. There were no any toxic effects or mortality observed during the entire treatment period. The microscopic examination of histological slides of the vital organs and uterine morphometric analysis did not reveal any significant difference but showed normalcy as compared to controls. Results of the study show that intra-uterine infusion of RISUG did not show any adverse effects during 14 days toxicity study in rat, thus, indicate that it is safe to use for further studies.

Keywords: RISUG, Intra-uterine infusion, Toxicity profile, Uterine morphometry, Rats.


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