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Abstract

OPTIMIZATION OF L-ASPARAGINASE PRODUCED BY OSCILLATORIA TEREBRIFORMI ISOLATED FROM MEDITERRANEAN SEA COAST, EGYPT

*Reham G. Elkomy

ABSTRACT

In this experiment cyanobacterium Oscillatoria Terebriformis was isolated from Eastern harbor Alexandria in Mediterranean coast of Egypt. The highest enzyme productivity was observed on the 12th days and the optimized parameters were pH 7, 30oC and 3000 lux. Immobilization using adsorbed on Luffa pulp enhanced the enzyme production compared to the conventional free- cells repeated reuse of the adsorbed cells achieved the maximum enzyme specific activity (55.562U/ml).

Keywords: Marine cyanobacterium; optimization; asparaginase; production.


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