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Abstract

IN VITRO ANTIOXIDANT AND ANTI-INFLAMMATORY PROFILES OF BIOACTIVE FRACTION FROM OPILIA CELTIDIFOLIA (GUILL. & PERR.) ENDL. EX WALP (OPILIACEAE)

Konaté Kiessoun*, Méa Arsène, Kassi Yomalan, Oksana Sytar, Alain Souza, Marian Brestic and Mamoudou H. Dicko

ABSTRACT

Polyphenol contents, antioxidant and anti-inflammatory activities of bioactive fraction (ethyl acetate fraction) of (leaves, leafy stems and roots) from Opilia celtidifolia were investigated in this study in oder to provide a scientific basis for the traditional use of plants in Burkina Faso. Folin-ciocalteu, aluminium nitrate using rutin as the standard methods respectively were used to access phytochemical composition. The antioxidant potential of the samples was evaluate using three separate methods, hydroxyl radical scavenging assay, hydrogen peroxide scavenging assay and phosphomolybdate assay for the total antioxidant capacity of ethyl acetate fraction. For anti-inflammatory activity, the Human Red Blood Cell (HRBC) membrane stabilization method, Membrane stabilization test by heat induced hemolysis, proteinase inhibitory activity and lypoxygenase inhibition methods were used. In this study, the ethyl acetate fraction of leaves of O. celtidifolia exhibited the best results.

Keywords: Polyphenol, antioxidant, anti-inflammatory, bioactive fraction.


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