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Abstract

ANTI-PROTEASE ACTIVITY AND HEAT STABILITY PROPERTY OF PIPER LONGUM SEEDS EXTRACT

Chikanna D., Dinesha R., Santhosh Kumar N.*, Manjula R. V., Tejaswini BM and Divya Y.

ABSTRACT

Long pepper (Piper longum) also called as Indian long pepper (pipli) belongs to the family of Piperaceae extensively used as spice and as medicine in South Asian continent. Herein we are reporting the property of inhibiting the activities of proteases like trypsin and chymotripsin by the crude protein of Piper longum. It exerts protease inhibitory activity up to 68% at 25 μg crude protein dose against Serine proteases. The protein was stable up to a temperature of 700C, active over a wide range of pH from 2 to 12. This is the first report on protease inhibitor property of Piper longum crude protein.

Keywords: Piper longum, Protease inhibitors, trypsin and chymotrypsin.


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