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Abstract

A DEVELOPMENT AND VALIDATION OF AN RP-HPLC METHOD FOR ESTIMATION OF FENOPROFEN IN BULK DRUG

Syed Rihana*, Dr. A. Suneetha, G. Sandhyarani, G. Sumanjali, G. Jyothi, G. Chaitanya

ABSTRACT

Rapid and sensitive method was developed and validated for the estimation of fenoprofen calcium in bulk drugs. Liquid-liquid extraction with methanol and acetonitrile. The accuracy and reproducibility of the method were within acceptable. Linearity ranges from 10 to 80 microgram/ml .the run time of the sample is 10 minutes.selected wavelength of the drug is 270nm. Operating temperature at 25degree celcius (ambient) by using column –enable c18 (4.6×250mm, 5 micrometers). Limit of the developed rp-hplc method for the estimation of fenoprofen, carried out on with enable c18 (250nm×4.6, 5μm) column. Methanol and acetonitrile in the ratio (80:20v/v) used as mobile phase and flow rate 1.5 ml/min. The detection was carried at 270nm and ambient column temperature was maintained.

Keywords: linearity, accuracy, precision, limit of detection, limit of quantification.


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