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Abstract

HPTLC FINGER PRINTING ANALYSIS OF THE TANNINS FROM AILANTHUS EXCELSA (ROXB.) LEAVES

Sudhir Singh Gangwar, Amita Tilak, Ranjana Sharma and Ravindra C. Sutar*

ABSTRACT

Objective: The present study was conducted to identify the tannins from petroleum ether (PEAE) extract of medicinally and economically useful leaves of Ailanthus excelsa (Roxb.) using High Performance Thin Layer Chromatography (HPTLC) technique. Methods: Preliminary phytochemical screening was done and HPTLC studies were carried out. CAMAG HPTLC system equipped with Linomat V applicator (Switzerland). Densitometric scanning was performed with Camag TLC scanner IV in the reflectance absorbance mode at 540 nm and operated by Win CATS software (1.4.6 Camag) with the help of tungstant lamp. Results: HPTLC finger printing of tannins of petroleum ether extract of leaves revealed eight polyvalent phytoconstituents (8 peaks) and corresponding ascending order of Rf values in the range of 0.005 to 0.935. Conclusions: With the results of HPTLC analysis and above Rf values we have concluded the presence of tannins in the extract.

Keywords: Ailanthus excelsa (Roxb.) leaves, tannins, HPTLC Fingerprinting.


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