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Abstract

COMPARATIVE STUDY ON ANTIOXIDANT ACTIVITY OF ESSENTIAL OILS FROM POGOSTEMON BENGHALENSIS (BURM.F.) KUNTZE. AND P.CABLIN (BLANCO) BENTH

Pradeep D. P.* and K. Murugan

ABSTRACT

Essential oils (Eos) are complex mixtures of volatile lipophilic
components and are obtained from leaf, twigs, fruits, flowers of higher
plants. These oils are isolated commonly through hydrodistillation.
Pogostemon species are well known for their essential oils and
therapeutic values. Chemical composition and antioxidant activity of
Eos from two species viz. Pogostemon benghalensis and P. cablin
were evaluated. Essential oil from both species was extracted by
hydrodistillation using Clevenger type apparatus. The essential oil
obtained was subjected to GC-Fid analysis followed by GC-MS. The
antioxidant activity was studied using DPPH, ABTS, FRAP, metal
chelating, hydrogen peroxide, super oxide radical, reducing power,
hydroxyl radical scavenging assays using ascorbic acid and butylated hydoxytoluene as
standards. 36 to 41 compounds were identified from the essential oil of Pogostemon species.
α-Cadinol (35.78%) and patchouli alcohol (34.85%) were the major components in these oils.
In addition to these compounds 1,8 cineole, aromadendrene, β-patchoulene, α-caryophyllene,
β-caryophyllene, α-patchoulene, germacrene A were identified as the other predominant
compounds. The antioxidant activity of Eos was significant when compared with standards.
75.3 to 91.5% inhibition activity was noticed against various free radical scavenging assays.
The Eos showed remarkable IC50 value which suggests their potency to use as a natural
antioxidant in various industries, foods and therapeutics.

Keywords: Essential oils, Pogostemon, hydrodistillation, GC-MS, ?-Cadinol, patchouli alcohol.


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