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Abstract

EVALUATION OF ANTIBACTERIAL ACTIVITY AND CYTOTOXICITY OF ROOTS OF WALTHERIA INDICA

*Dr. Rajaa H. Tabidi, Dr. Esam A. Babeker and 3Dr. Raga E. Osman

ABSTRACT

Background and Objective: The aim of this study was the determine in vitro antibacterial activity of different extracts of Waltheria Indica roots against standard bacteria, determine the Minimum Inhibitory Concentrations (MICs), study the cytotoxic effect of active extracts against different cell line and identify the major chemical components of the plants extracts. Material and Method: The cup agar plate diffusion method was used to screen the antibacterial activity of plant extracts and antibiotic. The Minimum Inhibitory Concentrations (MICs) of the most active extracts were determined using the agar plate dilution method. Micro-culture-tetrazolium MTT-assay was utilized to evaluate the cytotoxicity of extracts. All data were expressed as mean ± standard Deviation mean. Analysis of variance was performed by ANOVA using the SPSS software. Significant differences between means were determined by Least Significant Difference (L.S.D) and student t-test. A significant difference was considered at the level of P <0.05. Results: The results indicated that most extracts exhibited inhibitory effect against stranded bacteria. The lowest minimum inhibitory concentration (MIC) values was <3.125 mg/ml. Ps.aeruginosa, E.coli, S.aureus and B.subtilis were sensitive to Amoklan at concentration 100μg/ml, while S.aureus was resistant. Most extracts inhibited organism more than drug used. Alkaloid, flavonoids, tannins, saponins, sterols and triterpenes were found in extracts. The results of Micro-culture-tetrazolium (MTT) assayrevealed that all extracts tested were non-toxic.

Keywords: Antibacterial, Cytotoxicity, Waltheria Indica, MTT-assay, Roots, Fraction


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