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*Dr. Rajaa H. Tabidi, Dr. Esam A. Babeker and Dr. Sallwa Osman ELlaithi


Background and Objective: The aim of this study was the determine in vitro antibacterial activity of different extracts of Moringa oleifera leaves against clinical isolates and standard bacteria, determine the Minimum Inhibitory Concentrations (MICs) and identify the major chemical components of the plants extracts. Material and Method: The cup agar plate diffusion method was used to screen the antibacterial activity of plant extracts and antibiotic. The Minimum Inhibitory Concentrations (MICs) of the most active extracts were determined using the agar plate dilution method. All data were expressed as mean ± standard Deviation mean. Analysis of variance was performed by ANOVA using the SPSS software. Significant differences between means were determined by Least Significant Difference (L.S.D) and student t-test. A significant difference was considered at the level of P <0.05. Results: The results indicated that most extracts exhibited inhibitory effect against stranded bacteria and isolates. The minimum inhibitory concentrations (MICs) ranged between 25 mg/ml and 50mg/ml for all the organisms. The methanolic extract of Moringa oleifera leaves is active against the tested gram positive and gram negative microorganism. The phytochemical screening reveal the presence of flavonoids, sterols, coumarins tannins, alkaloids, saponins, triterpenes and anthraquinones.

Keywords: Antibacterial, Phytochemical, Moringa oleifera, Extract, leaves.

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