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Abstract

ANTIINFLAMMATORY AND WOUND HEALING ACTIVITY OF HIPPEASTRUM PUNICEUM BULB EXTRACT

Beena Briget Kuriakose*, Deepa C. P. and Eva Lobelle Sampayan

ABSTRACT

The present study was undertaken to evaluate the folkloric claim of H.puniceum bulbs for its anti inflammatory and wound healing activity. Aqueous extract of H.puniceum bulbs was prepared and used for the study. In vitro anti-inflammatory activity was evaluated by protein denaturation method and proteinase activity inhibition method using Diclofenac 100-500μg/ml as the positive control. An ointment prepared using the aqueous extract of H.puniceum bulbs was used for the in vivo studies. In vivo anti-inflammatory activity was evaluated by the carrageenan induced rat paw oedema model using Diclofenac ointment as the positive control. Wound healing activity was studied by the excision wound model using Betadine ointment as the positive control. The test extract exhibited significant anti-inflammatory activity in vitro and in vivo. The extract was also found to produce significant wound healing activity compared to the control. The results of the study underline the folkloric claim of H.puniceum in wound healing and reducing inflammation.

Keywords: H. Puniceum bulbs, Antiinflammatory Activity, Wound Healing Activity, Betadine, Diclofenac.


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