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Abstract

INHIBITORY EFFECT OF ETHANOLIC EXTRACT OF CITRUS PARADISI AND NARINGIN ON LIPID ACCUMULATION IN 3T3-L1 CELL LINES

R. Roghini and K. Vijayalakshmi*

ABSTRACT

Background: The aim of the study is to examine the effects of Naringin and ethanolic extract of Citrus paradisi on lipid accumulation in 3T3-L1 cell line. Materials & Methods: Various tests such as MTT assay, Lipid accumulation assay by oil red O staining, Cell proliferation assay, Measurement of free Triglycerides content, Glycerol 3 Phosphate dehydrogenase was assessed Mean standard deviation (SD). One-way analysis of variance (ANOVA) was used. The significance of differences between two groups (control and sample) was analyzed by the Student’s t-test. Results: Study findings show that the lipid accumulation was significantly reduced with all concentration of Citrus paradisi and Naringin compared with the control; Citrus paradisi and Naringin treatment on 3T3-L1 pre-adipocytes during proliferation decreased the cell viability in all treated groups was noted. Among the concentrations tested, 500μg/mL significantly reduced the viability of 3T3-L1 cells. There was a highly significant decrease in TG level at 250μg/ml, 500 μg/ml, and 750 μg/ml (P<0.001) dose of Citrus paradisi in comparison to controls. Naringin and Citrus paradisi inhibited GPDH activity in 3T3-L1 adipocytes in all treated groups. Conclusion: The lipid deposition and maturation of 3T3-L1 preadipocytes into adipocytes is prevented by treating with different doses of Citrus paradisi and Naringin.

Keywords: Citrus paradisi, Naringin, 3T3-L1 preadipocytes, GPDH activity, MTT assay.


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