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Abstract

UV-VISIBLE SPECTROPHOTOMETER METHOD DEVELOPMENT AND VALIDATION OF CITICOLINE IN SYRUP FORMULATION

Atif Ali*, Anza Sulemani, Awais Shabir, Muhammad Naseem, Sayed Muzahir Hussain, Muhammad Kamal Khan and Abdul Basit Muhammad

ABSTRACT

An accurate, simple and specific UV-Vis spectrophotometer method has been developed for the quantitative estimation of citicoline in bulk syrup. The wavelength was set to 280 nm. The correlation coefficient of citicoline (0.9990) and the regression equation (y = 1.0302×-2.2688) were shown by a linear calibration curve ranging from 16 μg/ml to 24 μg/ml. The %RSD result is less than 2%. The method has been validated against USP and ICH guidelines to verify method validation parameters such as linearity, accuracy, recovery and robustness. Therefore, a validated method can be used to quantify citicoline in the form of bulk syrup.

Keywords: Calibration curve, Analytical Method, Citicoline, UV spectroscopy.


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