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Abstract

A REVIEW: DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR QUANTITATIVE ANALYSIS OF PHARMACEUTICAL DOSAGE FORM

Vare S. R.*, Shelke M. M., Gholap S. M., Bidkar J. S. and Dama G. Y.

ABSTRACT

RP-HPLC technique is a simple, Precised, Accurate method for quantitative analysis of API and pharmaceutical dosage form. High Performance Liquid Chromatography which is also known as High Pressure Liquid Chromatography. This technique involves two phases stationary and mobile phases. The separation of constituents is based on the difference between partition coefficients of the two phases. The interaction of each sample component will be varies and this causes difference in flow rates of each component and finally leads to separation of components. This review mainly focuses on the HPLC technique, its principle, types, instrumentation, applications and method development and validation of method.

Keywords: RP-HPLC, Method development, Validation.


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