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Abstract

DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR QUANTITATIVE ANALYSIS OF ZOLEDRONIC ACID IN PURE AND PHARMACEUTICAL FORMULATIONS

P. Jitendra Kumar*, Ch. Sharmi Prakash, Ch. Venkatesh, G. Gopi Reddy, G. Satish, D. Rama Brahma Reddy

ABSTRACT

Simple reverse phase high performance liquid chromatography (RPHPLC) method has been developed for the estimation zoledronic acid in pure and pharmaceutical formulation. The separation was done on a 5μ c18column (250×4.6mm) using a mobile phase that consist of the phosphate buffer(pH adjusted ortho phosphoric acid 3.5) and methanol in the ratio of 96:4 (v/v). The flow rate was maintained at 0.7 ml/min The detection was done at 210nm using a uv detector. The retention times of zoledronic acid is 7.8 respectively. The method was validation done according ICH parameters.

Keywords: Zoledronic acid, orthophosphoric acid, rp-hplc, column.


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