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Abstract

VALIDATION METHODS OF DAPSONE DRUG WITH COMPARITIVE STUDY OF STANDRAD AND SAMPLE BY USING UV-SPECTROSCOPY

*Chintala Parimala Devi, Annapurna K., Govardhani P., Habeebunnissa K., Sowjanya K., Babaiah R., Mahesh A. and Uday kumar B.

ABSTRACT

The present study of this article is validation methods of dapsone with comparative studies of crude form by using UV-Spectroscopy. During the validation process the drug will studied with comparatively. The absorbance ranges from 294 to 296 nm. UV is the most effect method for the validation studies for comparison purposes. The process which is used in the validation methods of this article will obey the beers lamberts law. The method was validated in terms of Linearity, Precision, Accuracy, Ruggedness, Robustness, LOD, LOQ and Assay. Results of analysis were validated statistically and by recovery studies. From that it was observed that there was no interference of impurities or excipients during the validation of drug in formulation. Further this method utilizes no additional chemical reagents for development of any coloring species with Dapsone as observed in some studies in literature and hence this method is very accurate, simple and less time consuming. This shows adaptability of the method for validation of dapsone in crude and dosage form.

Keywords: Dapsone, validation, UV-spectroscopy, comparative study, analysis, first order.


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