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Abstract

EFFICACY OF CYPERUS ROTUNDUS RHIZOMES-TUBERS EXTRACTS AGAINST PROTOSCOLECES OF ECHINOCOCCUS GRANULOSUS

*Shnawa B.H. Gorony Sh.M. and Khalid K.M.

ABSTRACT

Background: Cystic echinococcosis is a zoonotic disease with increasing public health concern. Dissemination of protoscoleces –rich fluid during surgery is a major cause of recurrence, so injection of scolicidal agent into hydatid cyst to reduce the risk of spillage of viable protoscoleces is an integral part of the surgical operation. Objectives: To determine the in vitro protoscolicidal effect of Cyperus rotundus rhizomes-tubers extracts against Echinococcus granulosus. Materials and methods: C.rotundus rhizomes-tubers were collected from Bardbar village, Erbil, Kurdistan, Iraq. Freshly isolated protoscoleces were incubated with (500, 100,20,4 mg/ml) of aqueous and alcoholic extracts. Protoscoleces were tested with 0.1%eosin stain; also treated protoscoleces were processed for scanning electron microscopy compared to control one. Results: The effect on protoscoleces was investigated by eosin staining, light microscopy, and electron microscopy. The maximum effect was found in concentration of 500 mg/ ml of both extracts, mortality reached 100% after 2 hrs. post- incubation. The effect was seemed to be dose and time dependent with little differences between the two types of extracts. Results of viability tests were consistent with protoscoleces alteration and damage observed under both light and scanning electron microscopy. The first site of damage was the tegument of the parasite and the alteration included rostellar disorganization, loss of hooks and calcareous corpuscles, loss of turgidity of the outer membrane, and formation of the blebs on the tegument. Conclusion: The data recorded in this article demonstrate for the first time a clear in vitro scolicidal effect of C.rotundus rhizomes-tubers extracts against E.granulosus protoscoleces, however, in vivo effect of these extracts need to be examined.

Keywords: Cyperus rotundus, Protoscolicidal, Echinococcus granulosus, Ultrastructure.


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