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Abstract

BIOPLASTIC (POLY -3-HYDROXYBUTYRATE) PRODUCTION BY LOCAL PSEUDOMONAS AERUGINOSA ISOLATES UTILIZING WASTE COOKING OIL

Dr. Iman H. Gatea*, Nadhim H. Haider and Saad H. Khudair 

ABSTRACT

Disposal of domestic wastes, such as waste cooking oil (WCO), without pretreatment, contributes to the deterioration of the environment and creating sever problems. Synthetic plastic poses threat to the environment, because it is non-biodegradable are now accumulate in the environment at great millions of tons per year. Bioplastic are polymers such as Poly -3-hydroxybutyrate (PHB), which has gained importance since it can be easily degrade in nature. The present study was aimed to isolate a promising microbial producer of (PHB), and optimization experiment to evaluate the best environmental and physiological factors that lead to maximum (PHB) production. Seventy Pseudomonas species were isolated from different contaminated soil and water samples. The isolates thereafter were screened for (PHB) production using Sudan black and Nile blue as indicators stains to investigate the bacterial isolates for PHB production. It was found that 50 of them were capable of producing PHB. The isolate Pseudomonas aeruginosa Dw7 exhibit higher production of PHB, therefore it's selected for further studies. . Pseudomonas aeruginosa D7 was showed maximum production after 48 hours of growing on WCO yielding 1.74g/l of dry weight and 0.62g/l of PHB. Deferent WCO were tested as the sole carbon sources, the isolate yielded a relatively good dry weight 1.99g/l with 0.75 of PHB yielding 37% (PHB/dry weight of biomass) when grown on corn oil waste with initial concentration of 2%. Ammonium sulphate was found to be the best nitrogen source which raises the production of dry weigh biomass to 2.67 g/l with 1.03 g/l of PHB (yielding 39 %). The highest (PHB) yield was observed as an intracellular product in Pseudomonas aeruginosa Dw7 accumulating as high as 4.32 g\l dry weigh with 2.30 g/l of PHB (yielding 53.2 %) in C\N ratio of 30:1 in culture medium. Using WCO as a carbon source for synthesis of PHB is an economic process, turns polluting wastes into a valuable biodegradable product. This renewable source of waste oil can thus be exploited as low cost materials for PHB production.

Keywords: Bacteria; Poly-3-hydroxybutyrate; Oil waste; Bioplastic.


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