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Abstract

EVALUATION OF ANTIBACTERIAL ACTIVITY AND PHYTOCHEMICAL SCREENING OF THE CRUDE EXTRACT OF NIGELLA SATIVA SEEDS ON THE BACTERIAL ISOLATES OF WOUND

Usman M, Kabiru M*, Manga S. B., Opaluwa S. A., Nataala S. U., Garba, M. K., Spencer T. H. I., Nura A. and Bukar A.

ABSTRACT

Background: Nigella sativa (Black cumin, Habbatussawda) is a medicinal herb used in the treatment of many infectious and non infectious ailments. Objective: To investigate the effect of the antibacterial activities of the crude extracts of this plant was compared against that of Ciprofloxacin and Pefloxacin on seven wound associated bacteria. Materials and Methods: This study was conducted as cross sectional experimental study. The susceptibility pattern of the crude extract, fractions and the standard antibiotics were determined using Kirby-Bauer disc diffusion technique. MIC of the extract, fractions and the standard antibiotics were determined using Macro broth dilution techniques. Separation of the crude extract was carried out using column chromatography and the LD50 of the extract was determined using Lorke’s method. Data were analyzed using SPSS version 20.0 (California, Inc., USA) and p < 0.05 was considered significant. Results- Highly significant antibacterial activity was observed with the Methanolic seed extract on Coagulase negative Staph (at 10, 5 and 1mg/disc, F = 0.001, F = 12.4). No any significant activity was observed with the crude diethyl ether extract at all concentrations. The most highly insignificant activity was observed on P. aeruginosa (F = 0.074, P = 2.10). Similarly, no any significant difference was observed between the MICs and MBCs of the crude Methanolic seed extract when compared with that of Ciprofloxacin. An oral acute toxicity studies had shown an LD50 of the crude Methanolic seed extract to be >5000mg. Conclusion: Findings of the present study revealed that the overall result of the present study provided evidence that the crude extracts of Nigella Sativa seeds as well as some of the fractions (especially TF5; fifth fraction of N.sativas) could be considered as a potential source of novel antibacterial agents which may be employed to forestall the present antibiotic resistance menace pending further work on drug development processes.

Keywords: Antibacterial activity. Nigella Sativa Seeds, Bacterial isolates of Wound.


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