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Abstract

CYTOTOXICITY EVALUATIONS OF THE AQUEOUS EXTRACTS OF HERBAL MELANIN DRIVED FROM NIGELLA SATIVA SEEDS USING IN VIVO AND IN VITRO MODELS TESTS

Omar Abasher Al-Tayib*, Samia Mohamed El-Badwi and Amel Omer Bakhiet

ABSTRACT

Background: Many studies over millennia have been carried out to
determine a widely used medicinal plant throughout the world. Of
these, most of the therapeutic properties of Nigella sativa (N. sativa)
seeds are due to the presence of thymoquinone which is major
bioactive component of the essential oil outcome, whereas melanin
was unknown to exist in N. sativa before despite the fact that the seeds
have a widely used medicinal plant throughout the world still need
more studies. Objective: To investigate the cytotoxic effect of aqueous
extracts of melanin extracted recently from N. sativa (NSM) seed coats
on in vivo and in vitro safety outcome and thus used as a new bioactive
contents and/or a promising natural therapeutic extract from a plant.
Materials and Methods: Wistar rats and experimental mice in vivo
tests with different NSM aqueous extracts doses according to their weight through
intraperitoneal injection (i.p.) have been undertaken. Also, histopathological in vitro section
tests using rat’s liver, intestines, kidney and testes segments were also observed and LD50
weas calculated. Either, different doses of NSM for in vitro viability of cells performed on
Vero cells to analyses the cytotoxic activity of the extracts obtained. It was determined by
cell death assay using the 3-(4,5-dimetylthiazol-2-yl)-2,5 diphenyltetrazolium bromide
(MTT) colorimetric assay tests. Also, morphological changes and CC50 were obtained and/or calculated. The cytotoxic effects of NSM extracts in experimental animals found safely, inducing action of NSM extracts in dose-response curves for each cell. Results: study showed that NSM extracts were significantly high safety during cell growth in a dose-dependent manner without inducing damage of in vivo and in-vitro cells used. The obtained data indicated the possible therapeutic and safety actions of the NSM aqueous extracts and that might pave the ways of its using as new bioactive compound and/or a promising health extract derived from a plant. Conclusion: The present work is an effort to provide in vivo and in vitro detailed and safety margins in using melanin extracts of the literature on scientific researches of the pharmacy-toxicological guide and thus lightening presence of melanin from N. sativa seeds.

Keywords: Cytotoxicity, Melanin, Nigella sativa, Vero cells, MTT assay.


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