WJPR Citation New

  All Since 2011
 Citation  2903  2393
 h-index  27  24
 i10-index  68  60

Login

Best Paper Awards

World Journal of Pharmaceutical Research (WJPR) will give best paper award in every issue in the form of money along with certificate to promote research activity of scholar.
            Best Paper Award :
Dr. Muhammad Baqir MR Fakhrildin
Download Article: Click Here

Search

Track Your Article

Abstract

ROLE OF OSTEOPONTIN AND DIKKOPF RELATED PROTEIN - 1(DKK-1) AS DIAGNOSTIC MARKERS OF HCV RELATED HEPATOCELLULAR CARCINOMA

Abdellah A. Omar*, Ashraf T. Abd Elmouttaleb, Khaled M. Elsayed and Gamal M. Soliman

ABSTRACT

Background: Osteopontin (OPN) is an important tumor marker, since it presents as an immobilized extracellular matrix molecule in addition to present as a secreted form in body fluids involving plasma. Osteopontin levels in the plasma were found to be significantly higher in hepatocellular Carcinoma (HCC) patients than in healthy control individuals and also higher than in patients with chronic liver diseases. Dikkopf related protein-1 (DKK-1) is a diagnostic and prognostic serologic marker for early HCC. The DKK-1 mRNA and protein levels were found to be up regulated in early HCC. Objective: The aim of the present study is to evaluate the role of plasma OPN level and Dickkopf-1 (DKK1) as potential markers of HCC among HCV infected patients, compared to AFP. Also, its relationship with clinicopathological features of HCC patients. Study design: This is a retrospective case control study. Subjects and Methods: The study included 90 adult subjects; they were classified in to 3 groups.Group1: It included 30 patients with HCC metastases. Group 2: It included 30 patients with chronic liver disease (CLD) (chronic hepatitis C without HCC). Group 3: It included 30 apparently healthy individuals as a control group. Plasma Osteopontin and Dikkopf related protein -1(DKK-1) were measured by Enzyme-linked immunosorbent assay ELISA. Results: The mean of DKK1 in control, CLD, HCC was 1.28± 0.383, 1.37± 0.414, and 2.58± 0.510 respectively, and P value = 0.000 and there were highly statistical significant differences between the three groups (p < 0.001). The mean of OPN in control, CLD, HCC was 31.15± 15.031, 153.60± 93.931, 349.83± 183.912 respectively, P value = 0.000 and there were highly statistical significant differences between the three groups (p < 0.001). The mean of AFP in control, CLD, HCC was 1.110± 0.224, 34.06± 43.702, 4666.01± 3938.67 respectively, P value = 0.000 and there were highly statistical significant differences between the three groups (p < 0.001). DKK1 and OPN levels were significantly higher in metastasis cases P= 0.000 than non metastatic cases while AFP level was non-significant P= 0.424. Patients with large tumor size have significantly higher OPN levels p=0.025 while non significantly different as regard AFP and DKK1 levels. Conclusion: OPN and DKK1 can be used for diagnosis of HCC and differentiation between HCC and CLD. OPN and DKK1have higher sensitivity and specificity than AFP and can be used for early diagnosis. Combination between OPN and DKK1has increased both sensitivity and specificity for detection of of HCC.

Keywords: Hepatocellular carcinoma, chronic liver disease, Osteopontin, Dickopf related protein- 1.


[Full Text Article]

Call for Paper

World Journal of Pharmaceutical Research (WJPR)
Read More

Email & SMS Alert

World Journal of Pharmaceutical Research (WJPR)
Read More

Article Statistics

World Journal of Pharmaceutical Research (WJPR)
Read More

Online Submission

World Journal of Pharmaceutical Research (WJPR)
Read More