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Abstract

SPECTROPHOTOMETRIC ESTIMATION OF SULPHASALAZINE IN PHARMACEUTICAL FORMULATIONS

Mallapu E. Rani

ABSTRACT

The present validation of a developed a new spectrophotomctric estimation of sulphasalazine in pharmaceutical Formulations In this method, the oxidation of sulphasalazine drug by a known excess amount of cerium IV sulphate in acid medium, the unreacted cerium IV sulphate was treated with Iron II Sulphate, After 5 minutes the result ant Iron III slphate solution was treated with (1M) Ammonium thiocyanate, immediately it forms blood red colour of Iron III sulphate thiocyanate drug complex. This blood-red coloured complex formed under standardized conditions was measured at 545 nm against reagent blank Beer’s law was obeyed for 50-250  g/ml of sulphasalazine Results of analysis were validated statistically and recovery studies. The procedures described were successfully applied to the estimation of sulphasalazine in pharmaceutical formulations.

Keywords: Sulphasalazine spectrophotometric cerium IV sulphate, Iron III sulphate (ammonium ferrous sulphate Solution) Ammonium thiocyanate solution.


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