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Abstract

GENETIC POLYMORPHISM IN ADIPONECTIN GENE IN IRAQI PATIENTS WITH TYPE-II DIABETES MELLITUS

Walaa A. Mohammed*, Hameed M. Jasim and Esam N. Salman

ABSTRACT

Diabetes is a chronic disease that occurs when the pancreas does not produce enough insulin or when the body cannot effectively use the insulin it produces. This study was conducted to investigate the relationship between genetic polymorphism in adiponectin gene and the incidence of Type-II diabetes mellitus (T2DM) in a sample of Iraqi patients. Genetic polymorphism was studied in the variation site rs2241766 of adiponectin gene (ADIPOQ) located on the long arm of chromosome three (3q27), which regarded as a risk factor for the incidence of T2DM. First of all, genomic DNA was extracted from blood samples for T2DM patients and healthy controls with a concentration ranged between 200-400 μg/ml and purity ranged between 1.5-2.0, then exon 2 of ADIPOQ was amplified using specific primers. Results of electrophoresis on 2% agarose gel for the amplified products showed that there is an amplified fragment with a molecular size 372 bp was obtained. Results of sequencing for the amplified fragment showed that 60% of healthy controls are (TT) non-polymorphic in the site of variation of exon 2 when aligned with the reference sequence in NCBI data base, while it was found that 40% of healthy controls are polymorphic heterozygous (TG) in the same site. Results also showed that 67.6% of T2DM patients are (TT) non-polymorphic in the same site of ADIPOQ exon 2, while 33.3% of those patients were polymorphic heterozygous (TG), and there is no significant difference between the homozygous variations in both groups of healthy controls and T2DM patients which refers that this polymorphism was not regarded as a risk factor for the incidence of T2DM in Iraqi patients under study.

Keywords: The metabolic syndrome, ADIPOQ, polymorphism, Type-II diabetes mellitus, case-control study, Iraq.


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