WJPR Citation New

  All Since 2011
 Citation  2903  2393
 h-index  27  24
 i10-index  68  60

Login

Best Paper Awards

World Journal of Pharmaceutical Research (WJPR) will give best paper award in every issue in the form of money along with certificate to promote research activity of scholar.
            Best Paper Award :
Dr. Muhammad Baqir MR Fakhrildin
Download Article: Click Here

Search

Track Your Article

Abstract

ISOLATION AND CHARACTERIZATION OF ANTIMICROBIAL PEPTIDES FROM DATURA INOXIA LEAVES HAVING ANTIMICROBIAL ACTIVITY AGAINST SELECTED BACTERIA

Samriti, Rajesh Biswas and Kakoli Biswas*

ABSTRACT

The rapid emergence of multidrug-resistant infections has presented a serious challenge to antibiotic treatment posing major health threat over the past decades. Antimicrobial peptides (AMPs) play an important role in host defense mechanisms and can be used as an alternative source to develop more potent antibiotic against drugresistant microbes. AMPs are ubiquitous and found in diverse organisms ranging from microorganisms to animals. AMPs have been described as an evolutionary ancient weapon against microbial infection. Plants are the precious source of natural antimicrobial molecules including antimicrobial peptides known as plant antimicrobial peptides (PAMPs). PAMPs exert multiple antimicrobial activities, which includes membrane permeabilization and interference with DNA, RNA and protein synthesis that might provide a suitable approach to prevent bacteria from developing resistance. The present research work was aimed at isolation and characterization of antimicrobial proteins from Datura inoxia leaf extract. Leaf extract of Datura inoxia showed antimicrobial activity against Staphylococcus aureus and Bacillus subtilis whereas no activity was observed against Escherichia coli and Candida albicans. Maximum zone of inhibition (10 mm) was found against S. aureus and minimum (2 mm) against B. subtilis using 95% protein pellet. In Tricine SDS-PAGE, six, four and two bands lower than 26.6 kDa were observed in dialyzed samples of 50%, 75% and 90% protein pellet of the extract respectively. Protein bands lower than 26.6 kDa were observed in all the above-mentioned protein pellet of the extract that showed antimicrobial activity. These protein bands are putative AMPs which may be used or modified to develop antibiotics against drug-resistant microbes.

Keywords: Plant antimicrobial peptides, Thionins, Defensins, Lipid transfer protein, Tricine SDS-PAGE gel electrophoresis.


[Full Text Article]

Call for Paper

World Journal of Pharmaceutical Research (WJPR)
Read More

Email & SMS Alert

World Journal of Pharmaceutical Research (WJPR)
Read More

Article Statistics

World Journal of Pharmaceutical Research (WJPR)
Read More

Online Submission

World Journal of Pharmaceutical Research (WJPR)
Read More