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Abstract

THE ROLE OF MORINGA OLEIFERA EXTRACT IN MAINTENANCE OF BRAIN AND TESTIS TISSUES AGAINST AFLATOXIN B1 (AFB1) TOXICITY ON LIGHT OF MOLECULAR GENETIC AND HISTOLOGICAL STUDIES IN RATS

Dalia M. Aboelhassan*, Naglaa A. Hafiz, Marwa E. Shabana, Inas S. Ghaly*, Ibrahim M. Farag, Aboelfetoh M. Abdalla and M. I. Ezzo

ABSTRACT

Moringa oleifera is a promising plant that accounts for the medicinal uses such as a vital antioxidants, antibiotics and nutrients including vitamins and minerals. The present study was conducted to quantify the genotoxic and histopathological effects of AFB1 treatment on brain and testis tissues of rats and investigate the protective and curative role of M. oleifera leaf extract (MOLE) against AFB1 toxicity in rats. 72 rats were involved in this study and divided into 9 groups (8 each), normal control (G1), control of DMSO (G2), positive control that injected with AFB1 in DMSO (G3), G 4 to G6 were injected with AFB1 in DMSO plus MOLE as a protective agent at doses 3.3, 4.0 and 4.7 mg/kg respectively, while G7 to G9 of rats received MOLE with the same three doses, post treatment with AFB1, as a therapeutic groups. Molecular genetic and histopathological assessment of brain and testis tissues and immunohistochemical studies were involved. Exposure to AFB1 caused significant elevation of DNA damage, upper-regulation of studied genes in brain and testis tissues, respectively and induced massive damage in cerebral cortex and cerebellum in brain tissues as well as testicular degeneration of seminefrous tubules in testis tissues that confirmed by immunohistochemical results. Protective or therapeutic treatment with MOLE significantly decreased the DNA damage, suppress the over expression studied genes and histopathological and immunohistochemical damage. Best findings were given with the highest dose of MOLE as curative agent in rats. Conclusion: MOLE effectively alleviates AFB1 induced over expression of 5a-R3 and LHR genes and improved the histopathological damages in brain and testis tissues in rats. The consumption of MOLE-basal diet could maintain the integrity of genetic materials and histological architectures against AFB1 toxicity.

Keywords: Aflatoxin B1, MOLE, genotoxicity, histological changes.


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