WJPR Citation New

  All Since 2011
 Citation  2903  2393
 h-index  27  24
 i10-index  68  60

Login

Best Paper Awards

World Journal of Pharmaceutical Research (WJPR) will give best paper award in every issue in the form of money along with certificate to promote research activity of scholar.
            Best Paper Award :
Dr. Muhammad Baqir MR Fakhrildin
Download Article: Click Here

Search

Track Your Article

Abstract

STABILITY INDICATING RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR THE SIMULTANEOUS ESTIMATION OF GEMCITABINE AND CLARITHROMYCIN IN BULK AND PHARMACEUTICAL DOSAGE FORM

Y. Anil Kumar*, Y. Sirisha, A. L. Harini and A. Prathyusha

ABSTRACT

A simple, accurate and precise Stability indicating RP-HPLC method for the estimation of in pure and pharmaceutical dosage form has been reported. Quantitative estimation of Gemcitabine and Clarithromycin was done by using WATERS HPLC 2965 SYSTEM with Auto Injector and PDA Detector on a Chromosil C18 Column (150mm x 4.6mm) 5μg.). A 10μL syringe was used for injecting the samples. Data was analyzed by using Empower 2 software. UV-VIS spectrophotometer shimadzu with special bandwidth of 2mm and 10mm and matched quartz was used for measuring absorbance for Gemcitabine and Clarithromycin solutions. The mobile phase consists of a water: phosphate Buffer: Methanol (35:65v/v) And at a flow rate of 1 milliliter/minute. Gemcitabine and Clarithromycin were eluted at approximately 7 minutes. The wavelength was found to be 254nm. A linear response was observed in the concentration ranges of 20- 60μg/ml with a regression coefficient of 0.999. Forced degradation studies were performed on pure sample of Gemcitabine and Clarithromycin using acid (0.1 Normal (N) hydrochloric acid), base (0.1 N sodium hydroxide), peroxide (30% H2O2) and thermal (105°C) conditions. The developed method was validated with respect to specificity, precision (% RSD about 0.4%), linearity (linearity of range about 20-60 μg/mL), robustness, LOD and LOQ values were found to be 0.001μg/ml, 0.005μg/ml and 0.004μg/ml 0.015μg/ml respectively.

Keywords: High performance liquid chromatography, Gemcitabine and Clarithromycin.


[Full Text Article]

Call for Paper

World Journal of Pharmaceutical Research (WJPR)
Read More

Email & SMS Alert

World Journal of Pharmaceutical Research (WJPR)
Read More

Article Statistics

World Journal of Pharmaceutical Research (WJPR)
Read More

Online Submission

World Journal of Pharmaceutical Research (WJPR)
Read More