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Srijana Kasturi*, A. V. S. Ksheera Bhavani, M. Madhuri and B. Ramadevi


The probiotic concept is having lots of different applications in human and animal health. Probiotics are the living organisms which, when administered in adequate amounts confer a health benefit on host. Probiotic products consist of different enzymes, vitamins, capsules or tablets and some fermented foods contain microorganisms. The beneficial effects produced by probiotics are like lactose intolerance, immune system, traveller’s disease, cancer, dysbiosis, cholesterol reduction, etc. The present research work is to study existing actinomycetes isolates for probiotic activity and have the surviving capability at low pH and bile tolerance towards bile salts. Among the existing isolates of VMS 20-30 isolates, VMS-30 was selected based on their antimicrobial activity tested against Staphylococcus aureus and Escherichia coli. Although in the stomach, pH can be as low as 1.0, in most in vitro assays pH 3.0 has been preferred. Due to the fact that a significant decrease in the viability of strains is often observed at pH 2.0 and below. As the existing actinomycete isolate was tested for their ability to tolerate the low pH, it was observed that there was no change in the growth of the inoculated organism. According to the results VMS-30 strains were resistant to 0.2%, 0.3%, 0.4%, 0.5% bile salt. The isolate was able to grow in bile salt as they survive.

Keywords: Probiotics, Bile tolerance, Acid tolerance, Actinomycetes.

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