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Abstract

RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR THE SIMULTANEOUS DETERMINATION ATENOLOL AND AMLODIPINE IN PHARMACEUTICAL DOSAGE FORMS

*Chandra Sekhar Kurimisetty, V. Surya Narayana Rao, Mastanaiah Thummisetty, I. Hemanth Kumar and P. Sai Kiran

ABSTRACT

A new, simple, precise, rapid and accurate RP-HPLC (Reverse Phase – High Performance Liquid Chromatography) method has been developed for the simultaneous estimation of Atenolol and Amlodipine in pharmaceutical dosage form. The chromatographic separation was achieved on Alliance Waters 2695 HPLC using HYPRSIL BDS, 150 x 4.6 mm, 5 column maintained at ambient temperature with mobile phase, Buffer: water: Acetonitrile (50:50), flow rate 1.0 ml/min, load volume 10 μl and a run time of 08 min. Buffer was prepared with Triethylamine and adjusted pH to 3.1 with Ortho-Phosphoric Acid. The retention time and mean recoveries obtained for Atenolol was 1.8 min and 100.04%, for Amlodipine was 6.0 min and 99.73.% respectively. Linearity response was established over the concentration range of 50-150 μg/ml for Atenolol and 5-15 μg/ml for Amlodipine. The Assay for Atenolol and Amlodipine was found to be 99.46 and 99.45 respectively. The recovery studies ascertained the accuracy of proposed method and the results were validated as per ICH guidelines. Hence, the developed method can be successfully employed for routine quality control of Atenolol and Amlodipine in drug testing laboratories and pharmaceutical industries.

Keywords: Atenolol, Amlodipine, RP-HPLC, Beta Blockers.


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