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Abstract

SPECTROPHOTOMETRIC ASSAY OF LANSOPRAZOLE IN BUILK AND DOSAGE FORMS

M. Ramesh Kumar*, M. Rama Reddy, U. Sreenivasulu and N. Rami Reddy

ABSTRACT

A simple, sensitive, rapid and accurate spectrophotometric method has been developed for the estimation of lansoprazole in pharmaceutical dosage forms. The method is based on the reaction of each drug having secondary or tertiary or primary amino (aliphatic) group with wool fast blue, the formed complex extracted into chloroform at pH 1.5. The absorbance of ion pair complex form in extractable chloroform layer is measured at the wavelength of maximum absorbance for each drug against the reagent blank (prepared in a similar manner devoid of drug solution) and the amount of drug is determined from the calibration curve made between the absorbance and the amount of drug. The amount of drug is computed from the calibration curve.

Keywords: Spectrophotometry, wool fast blue, lansoprazole Pharmaceutical and Formulation.


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