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Abstract

ANTICANCER ACTIVITY AND ANTI DIABETIC ACTIVITY OF STREPTOMYCES SPECIES JKCM1

*M. Guravaiah

ABSTRACT

The Actinomycetales, especially the genus Streptomyces, produce a number of antibiotics and other bioactive natural products. Measurement of cell viability and proliferation forms the basis for numerous in vitro assays of a cell population’s response to external factors. MTT Assay is a colorimetric assay that measures the reduction of yellow 3-(4,5-dimethythiazol- 2-yl)-2,5-diphenyl tetrazolium bromide (MTT) by mitochondrial succinate dehydrogenase. HepG2, Human Liver cancer cells were cultured in DMEM containing 4.5 g/L D-glucose with 10% heat-inactivated FBS at 37°C, 5% CO2 atmosphere.

Keywords: Actinomycetales, cell viability, colorimetric assay and MTT Assay.


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