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Abstract

A REVIEW ON LIQUID CHROMATOGRAPHY-MASS SPECTROSCOPY AND IT’S APPLICATIONS

*Shivam Mishra and Chandana Majee

ABSTRACT

The Liquid Chromatography-Mass Spectrometry (LC-MS) is an influential analytical technique with very high sensitivity and specificity. LC-MS is the combination of Liquid Chromatography (LC) and Mass Spectrometry (MS). With the Liquid Chromatography (LC) the separation of components can be done, and then the sample eluents from LC are transferred into Mass Spectrometry (MS) where the detection, identification, and determination of masses of components can be done in the presence of other elements. LC-MS is used in the resolution, of pharmaceutical drug substances, intermediates and its related compounds for the quantitative and qualitative purpose. LC-MS is used most significantly in bio-equivalence, in-vitro dissolution bioavailability and metabolite studies. Also, LC-MS is used in fundamental research, agrochemical, forensic laboratories and food industries. In this article principle of LC-MS, instrumentation and its applications are briefly discussed.

Keywords: Liquid chromatography (LC); Mass spectrometry (MS); Liquid Chromatography-Mass Spectrometry (LC-MS), bio-equivalence, in-vitro dissolution, bioavailability.


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