Abstract

EXPRESSION OF TUMOR SUPPRESSOR GENE P53 CORRELATION WITH GASTRIC ADENOCARCINOMA PATIENTS BY USING IMMUNOHISTOCHEMICAL ASSAY

Noor Al-Huda Ali A. H. Saeed*, Luma Qasim Ali, Areej Abbas Zabbon and Zaid A Saeed

ABSTRACT

Over expression of P53, tumor suppressor gene and alternation may involved in many types of human cancers, one of the most important kind is gastric cancer. In the current study, we shed the light on the relation between P53 and some aspects of gastric carcinoma patients by using immunohistochemical assay by taking 43 biopsies randomly from patients already have gastrectomy, samples were collected during the period from January 2015 until January 2016, including 26 patients above 50 years old and 17 under that age, male: female ratio 2: 1 with 30 men and 13 women. Histological grade including 21 well differentiated, 13 moderately and 9 poor differentiated types. According to TNM staging, 26 patients out of 43 falling in stages I and II, and the rest 17 patients were falling in stages III and IV. Whereas, the current study involved 27 smoker patients and 16 non-smokers. Here and by using immunohistochemical assay,P53 were detected in 28 out of 43 patients (65%), in the other hand, the positive results related in highly significant association to each of age, gender, histological grade, stage of the tumor and tobacco smoking of gastric carcinoma patients.

Keywords: gastric cancer, adenocarcinoma, p53, tumor suppressor gene, immunohistochemistry.