PHYTOCHEMICAL ANALYSIS AND INVITRO ANTIMICROBIAL ACTIVITY OF Alstonia scholaris R.BR. FLOWERS AGAINST ENDODONTIC PATHOGENS.
Ramya Madhiri*, Vijayalakshmi G., Ganga Rao Battu, Padma Dorothy K. and Jagadeesh Panda
ABSTRACT
Phytochemical analyses of hexane, ethyl acetate, chloroform and
methanol extracts of Alstonic scholaris flower was done. All the four
solvent extracts revealed the presence of anthocyanins, alkaloids,
terpenoids and coumarins whereas hexane, ethylacetate and chloroform
extracts revealed the presence of quinines and flavonoids. Phenols are
found in only hexane and methanolic extracts. tested against the oral
pathogens. Seven microorganisms, responsible for formation of plaque,
caries and oral infections, namely Streptococcus mutans, Enterococcus
faecalis, Lactobacillus acidophilus, Staphylococcus aureus,(Grampositive
bacteria). Pseudomonas aeruginosa, Escherichia coli (Gram-negative bacteria) and
Candida albicans (Fungi) have been selected to assess their susceptibility to various solvent
extracts of the flowers. The antimicrobial potential of the extracts was assessed by zone of
inhibition method and Resazurin microtiter assay (REMA) plate method. The zones of
inhibition obtained for the test organisms ranged between 11- 17mm diameter with the
extract concentration of 3mg/ml. These zones indicate that the growth of test organisms was
inhibited and they are susceptible to the extracts employed. MIC value of 1mg/ml of the
extracts inhibited 28-58% growth of test organisms. The results obtained indicate the
antimicrobial potential of flower extracts of Alstonia scholaris.
Keywords: Alstonia scholaris, Agar well diffusion method, REMA, Bioautography. MIC.
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