IN VITRO ANALYSIS OF ANTIFUNGAL ACTIVITY OF ALOE VERA EXTRACT AND ALOIN ON CANDIDA ALBICANS
Shobhna Tiwari* and Dr. M. K. Gupta
ABSTRACT
The aim of the study was to analyse, antifungal activity of Aloe vera
extract and aloin on candida albicans. 4 different solvents were used
for the extraction of Aloe vera which were extracted in petroleum
ether, chloroform, ethyl acetate and 70% methanolic extract.
Antifungal activity was tested by well diffusion assay. To determine
minimum inhibitory concentration(MIC), agar dilution and broth
dilution methods were performed. Each extract was taken in 4 varying
concentrations (100mg/ml, 150mg/ml, 200mg/ml and 250mg/ml) to
analyse the MIC by agar dilution method, which were further tested by
broth dilution method. Among extracts, 70% methanolic extract shows
maximum inhibitory action and extracts were compared with aloin which has shown more
potent antifungal activity than all the extracts, it is active ingredient of Aloe vera. To compare
the results fluconazole was taken as standard.
Keywords: Aloe vera, aloin, MIC, agar dilution, tube dilution.
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