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Abstract

UV-SPECTROSCOPY METHOD DEVELOPMENT AND VALIDATION OF DEFLAZACORT AND TAMSULOSIN HYDROCHLORIDE IN COMBINED DOSAGE FORM

Vijaykumar V. Rupapara*, Dr. Zarna R. Dedania and Dr. Ronak Dedania

ABSTRACT

 A simple, specific, accurate and precise First order derivative spectrophotometric method was developed and validated for simultaneous estimation of Deflazacort and Tamsulosin hydrochloride in combined dosage form. The wavelength of estimation for Deflazacort was 266 nm and for Tamsulosin hydrochloride was 242 nm. The linearity range was found to be 8- 40 μg/ml for Deflazacort and 1.6-8 μg/ml for Tamsulosin hydrochloride. The co-relation co-efficient was found to be 0.9916 and 0.9971 for Deflazacort and Tamsulosin hydrochloride respectively. The % recovery for Deflazacort and Tamsulosin hydrochloride were found to be 99.95-100.42% and 99.31- 100.31% respectively. Intraday precision of Deflazacort and Tamsulosin hydrochloride were found to be 0.87-1.46 and 1.33- 1.87% RSD and Interday precision were found to be 0.86-1.86 and 1.42-1.71% RSD respectively.

Keywords: Deflazacort, Tamsulosin hydrochloride, First order derivative UVSpectroscopy method, Validation.


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