ANALYSIS OF OXIDATIVE STRESS INDUCED BY DIFFERENT TOBACCO SAMPLES AND THE PROTECTIVE EFFECT BY CERTAIN PLANT EXTRACTS
Maji Jose, Vengal Ipe Varghese, Varsha Jayakar, Vinayak Lokapur, Srinivasa K., *Manjula Shantaram
ABSTRACT
The tobacco use has spread with significant rapidity percolating into all
sections of the society. Although tobacco usage is not a new problem,
it has become a source of increasing concern due to the increase in
realization of its volume, awful morbidity and mortality. Numerous
modes of action by which tobacco carcinogens induce cancer have
been recognised, including tobacco induced oxidative stress through
production of reactive oxygen species. Cellular antioxidant enzymes
such as superoxide dismutase, catalase and glutathione peroxidase and
free radical scavengers like reduced glutathione and vitamins A, C, and
E protect cells and tissues against noxious radicals. To analyze the
extent of oxidative stress that is induced by tobacco in different forms,
selected concentrations of alcoholic extracts of various tobacco products, ranging from 100-
300μgs were added to oral epithelial cell culture. Melondialdehyde and reduced glutathione
were estimated as a measure of oxidative stress and lipid peroxidation. Treatment of cell
culture with mango leaf extract, coconut husk extract and areca husk extract along with
tobacco samples have shown a significant variation in oxidative parameters such as
melondialdehyde and reduced glutathione levels and antioxidant enzymes such as superoxide
dismutase, catalase and peroxidase activity indicating protective effect.
Keywords: Oxidative stress, Tobacco, Mango leaves, Coconut husk, Areca nut husk.
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