ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF VALACYLOVIR HYDROCHLORIDE BY UV AND HPLC
Ashwini S. Pundkar* and Vilas A. Arsul
ABSTRACT
A Ultra-Violet (UV) and High Performance Liquid Chromatography
(HPLC) have been developed for the estimation of Valacyclovir
Hydrochloride in bulk drug and pharmaceutical dosage form. The
method is carried out using C18 column 150×4.6mm i.e. particle size
5μm and mobile phase consisting of Methanol: Water (70:30), at flow
rate of 0.8ml/min. The column temperature is ambient. Eluents were
monitored by UV detector set at 252nm. The method was statistically
validated in terms of linearity, accuracy, precision and robustness in
accordance with ICH guidelines Linear regression analysis data for the
calibration plot showed that there was a linear relationship between
response and concentration in the range of 10μgm/ml To 50μgm/ml and the correlation
coefficient is 0.9998. Literature survey reveals analytical methods for the estimation of
Valacyclovir Hydrochloride from pharmaceutical dosage forms and also in biological fluids.
The proposed method was found to be simple, precise, accurate, rapid and reproducible for
the estimation of Valacyclovir Hydrochloride in bulk drug and tablet.
Keywords: Valacyclovir hydrochloride, Method Development, UV and HPLC validation.
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