A MODIFIED RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR THE SIMULTANEOUS ESTIMATION OF NEBIVOLOL AND INDAPAMIDE IN BULK AND PHARMACEUTICALDOSAGE FORMS
T. Praveena* and S. Angala Parameswari
ABSTRACT
A simple, precise, accurate, reproducible and economical stabilityindicating
reverse phase liquid chromatography method was developed
and validated for the quantitative simultaneous estimation of Nebivolol
and Indapamide in bulk and marketed formulations. Estimation of
drugs in this combination was done with a C18 column Kromasil
column. 250mm × 4.6mm using mobile phase of composition
Acetonitrile and phosphate buffer (60:40 v/v, pH 3).The flow rate was
0.8 ml/min and the effluents were monitored at 226nm. The retention
time of Nebivolol and Indapamide were2.9 min and 4.2 min
respectively. The method was found to be linear over a range of 10-50
g/ml for Nebivolol and2-10 g/ml forIndapamide. The established method proved as
reproducible one with a %RSD value of less than 2 and having the robustness and accuracy
within the specified limits. Assay of marketed formulation was determined and find with
99.08% and 99.87% for Nebivolol and Indapamide respectively. The stressed samples were
analyzed and this proposed method was found to be specific and stability indicating as no
interfering peaks of degradation compounds and excipients were noticed. The method was
validated according to the guidelines of International Conference on Harmonization (ICH)
and was successfully employed in the estimation of commercial formulations. This liquid
chromatographic method can be applied for the qualitative and quantitative determination of
selected drugs by the modern chemist.
Keywords: Nebivolol, Indapamide, RP-HPLC, Stability and Method validation.
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