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Abstract

VIABILITY STUDY OF SOME ANTIBIOTIC RESISTANCE BACTERIA PERCENTAGE ISOLATED FROM WASTEWATER EFFLUENT SAMPLES IN MANISA / TURKEY

Shadman Tariq Sadiq* and ?hsan Ya?a

ABSTRACT

Antibiotic resistant bacteria and antimicrobial drugs enter the environment via wastewater, especially from hospitals and pharmaceutical plants, and through agricultural runoff, leading to contamination of surface and groundwater. Viability and percentage of antibiotic resistant bacteria (PARB) was investigated in effluent of urban house wastewater of Manisa city wastewater treatment plant system, the investigations done in university of Ege department of industrial microbiology. Samples of wastewater were collected monthly separated in two season (Spring & Summer) 2018, 1 liter in each sampling campaign filtered by aspiration pump with 0.45 μm filters. As a results of samples culturing in this study we noticed that samples for two season contain different levels of bacterial populations including Pseudomonas, E. coli, Salmonella and shigella that showed different growth rates among the examined samples. High numbers found in summer was 3.39E5, while lowest growth of both groups of bacteria was detected in water samples in June which was about 2.15E2. As results of antibiotics resistance percentage we found that all the isolates were resistant to ampicillin especially in month of Aug. was about 86% resistance of the total number of heterotrophic bacteria followed by the same antibiotic resistance percentage in July was about 67% and 51% for Amoxicillin in July between all of the total number of heterotrophic bacteria. In versa, the lowest antibiotics resistance percentage was with Kanamycin about 2% in the month of June also the resistance to the same antibiotic was not detected-ND in other samples for other months. Finally, we noticed that storing in cold conditions for interval between 10-14 days’ lead to reduction of ARB to 90% before effluent of WW to the environment.

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