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Sindhuja P. and Gomathi N.*


The present study was carried out to evaluate the Antimicrobial, Antioxidant, Cytotoxicity and Anticancer activity of Flaxseed extract. The two different solvents like water and ethanol were used for extraction. Antimicrobial activity was determined by Agar disc diffusion method and MIC. Aqueous and Ethanol extract of Flaxseed showed inhibition against Salmonella typhi and Pseudomonas aeruginosa and no inhibition effect was noticed against against Escherichia coli, Shigella species, Proteus species and Staphylococcus aureus. The flaxseed extract did not showed antifungal activity against the tested fungi –Aspergillus niger, Aspergillus flavus, Rhizopus species, Penicillum species and Candida albicans. Antioxidant activity was determined by DPPH and FRAP assay. Cytotoxicity was done on vero cell line and toxic free concentrations were estimated. Anticancer activity was done against HeLa cell lines by MTT assay. Cytotoxicity revealed that the flaxseed extracts were non-toxic and were capable of inhibiting the proliferation of HeLa cell line. Flax seed was found to contain Antibacterial, Antioxidant and Anticancer properties.

Keywords: FLAXSEED, Antioxidant activity, DPPH assay, FRAP assay, MTT assay, HeLa cell line.

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