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Manonmani R.*, Catharin Sara S., Revathi M. and Basheera Begam M.


The present investigation was carried out in the plant Nigella sativa L. The seeds of N. sativa L. were screened for the presence of biologically active compounds by suitable methods. The present investigation deals with the study of phytochemical profiling of a medicinal plant N. sativa L. which covers the screening of preliminary phytochemical compounds, analyzing the phytoconstituents using GCMS, identifying the functional groups using FT-IR and absoption range using UV-VIS. The study was undertaken to identify and analyze the phytochemical constituents which may be responsible for the development of new drug. The preliminary phytochemical analysis showed the presence of major phytoconstituents like alkaloids, phenolic compounds, flavonoids etc. The studies on the bioactive components in the methanolic seed extract of N. sativa L. by GC-MS analysis clearly showed the presence of four bioactive compounds such as 2-hexyldecanoic acid (220.19%), 2-Hydroxy – 14 – methyl – oxa – cyclotetradec – 6 – en – 2 – one (122.04%), 11-Hexadecyn – 1 –ol (622.56%), 6, 9 - octadecadienoic acid methyl ester (226.27%). Infra-red spectrum of methanolic and aqueous seed extracts of N. sativa L. showed the presence of alkyl halide, alcohol, aromatic, acid, alkyne functional group. The results of UV-VIS spectrum analysis for both methanolic and aqueous seed extract of N. sativa showed the absorption at 311 nm (-0.403) and 310 (-0.110) respectively.

Keywords: Nigella sativa L., phytochemical profiling, GC-MS, FTIR, UV.

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