Abstract

COMPARATIVE STUDY ON THE EFFECT OF NANO-CURCUMIN COMPLEXES AS ANTITUMOR IN MICE

Radwa Wahid Mohamed, Tahani Elsayed Mohamed, Gehan Salah Eldin Moram*,
Abdelfattah Mohsen Badawy and Mohamad Fahmy Zaky

ABSTRACT

This study was undertaken to compare the antitumor activity of copper
oxide nanoparticles (CuO-NPs), hydrazine sulphate-copper
nanoparticle (HS-Cu-NPs), nano-curcumin (Nano-Cur), curcumin
capped copper nanoparticles (Cur-Cu-NPs) and native curcumin
(Native-Cur) with ascorbate in vivo and in vitro using breast carcinoma
cell line (MCF-7). Methods: CuO-NPs and HS-Cu-NPs were injected
intratumorally at dose 10 mg/Kg b.wt., Cur-Cu-NPs was injected
intratumorally at dose 50 mg/Kg b.wt., Nano-Cur and Native-Cur were
injected intratumorally (G6, G7) or administered orally (G8, G9) at
dose 50 mg/Kg b.wt. In vivo, Female mice were divided into 9 groups:
G1: Healthy control. G2: tumor bearing mice (TBM). G3:
TBM+CuO-NPs + ascorbate. G4: TBM+ HS-Cu-NPs + ascorbate. G5: TBM+Cur-Cu-NPs +
ascorbate. G6, G8: TBM+Nano-Cur + ascorbate G7, G9: TBM+Native-Cur +ascorbate. In
vitro, Cell lines were treated with the different nano complexes for 48h. Results: In vivo,
Different nano-complexes significantly reduced tumor markers and inflammation
immunologic markers and significantly elevated the caspase-3 activity and P53 gene
expression. These results were confirmed by histopathological examination and
immunohistochemistry. In vitro, all nano-complexes showed a clear cytotoxic effect on
(MCF-7). In conclusion: All nano-complexes administration significantly inhibited tumor
growth and reduced tumor markers but Cur-Cu nano-complex was the most effective
treatment.

Keywords: Curcumin, Nanocurcumin, Curcumin capped copper nanoparticles, EAC, tumor bearing mice.