Abstract

A COMPARATIVE STUDY OF ANTI INFLAMMATORY AND ANTIOXIDANT ACTIVITIES OF FENUGREEK (TRIGONELLA FOENUM GRAECUM) AND CARAWAY (CARUM CARVI) AQUEOUS AND OIL SEED EXTRACTS IN EXPERIMENTAL MODEL OF ULCERATIVE COLITIS

Mai Elsayed* and Rasha H. Hussein

ABSTRACT

Ulcerative colitis is a chronic inflammatory bowel disorder that increase the risk of colon cancer. The usage of natural antioxidants exhibit great interest as a means to avoid oxidative damage. This study evaluated the effect of fenugreek and caraway either in form of aqueous or oil seeds extracts on experimental model of ulcerative colitis in rats. Colitis was induced using of 4% acetic acid solution. Groups were: G1: healthy control group, G2: ulcerative colitis group, G (3, 4) : ulcerative colitis supplemented with fenugreek aqueous and oil extracts, respectively, G (5, 6) : ulcerative colitis supplemented with caraway aqueous and oil extracts, respectively. Colon samples were assayed for oxidative stress and inflammatory biomarkers along with colon histology and DNA fragmentation. Results showed that treatment with fenugreek and caraway either in form of aqueous extract or seed oil extracts protect against ulcerative colitis effects as shown by improvement in antioxidant enzymes level and inflammatory biomarkers, histological and genetical evaluation. These findings indicated that fenugreek and caraway aqueous and oils extracts have defensive effects against acetic acid-induced ulcerative colitis which might due to their anti-inflammatory, antiulcerative and antioxidant properties. Therefore, they can possibly used for successful treatment of ulcerative colitis.

Keywords: Fenugreek - Caraway - Ulcerative colitis- DNA fragmentation -Antioxidant-Inflammation.