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D. P. Kawade* and N. P. Dubey


The determination of Metformin was carried out in pure and tablet forms. The drug was found almost stable to neutral and photolytic condition. Hence, the proposed method is suitable for application in quality-control laboratories for quantitative analysis of drugs individually. This research project deals with the studies on the “Development and validation of liquid chromatographic methods for the quantification of anti-diabetic drugs in formulations and biological samples. The need for drug analysis, chromatographic methodology (method development and optimization) and experimental results of drug quantification in dosage forms, biological matrix and validation of the HPLC method was used for single drug of antidiabetic class; Metformin as a development of the assay method. The mobile phase consisting of water and methanol in the ratio of 50: 50 at wavelength 239 nm, column C18 (phenomenex) and the flow rate 1 mL min−1and retention time 2.6 mins. A simple, rapid, accurate and stability indicating HPLC method was developed which is economic, sensitive and time saving than other chromatographic procedures. It is user-friendly and importance tool for analysis of combined dosage form. The developed method was successfully applied to the determination of metformin in pharmaceutical formulations.

Keywords: HPLC, Antidiabetic Drugs, Metformin etc.

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