ASSESSMENT OF DIFFERENT RESTRICTION ENDONUCLEASES: (SAL 1 AND XBA 1) ON PURIFIED CHLAMYDIA PNEUMONIA DNA
Otoikhian C.S.O.*, Okoror, L.E. Ekakitie, A.O.
Novena University, College of Natural and Applied Sciences, Department of Biological Sciences, Delta State, NIGERIA.
ABSTRACT
Two restriction endonucleases (Sal 1 and Xba l) where compared to
ascertain if same strain of Chlamydia pneumonia that is responsible
for respiratory tract infection of the patients isolated from Delta State
of Nigeria. Results revealed that 95 samples (76.61%), where positive
while 29 samples (23.38%) were negative using the culture method
and staining with giemsa technique. The restriction endonucleases Sal
l and Xba 1 cleaved the DNA at different point as evidenced on
agrose gel electrophoresis and also the DNA could not be cleaved by
both restriction endonucleases indicating strain variation in
Chlamydia pneumonia species.
Keywords: DNA, strain, Chlamydia pneumonia.
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