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Abstract

EFFECT OF NUTMEG SEEDS EXTRACTS (MYRISTICA FRAGRANS HOUTT) ON STAPHYLOCOCCUS AUREUS AND STAPHYLOCOCCUS EPIDERMIDIS

Sri Agung Fitri Kusuma*, Ronato and Ardian Baitariza

ABSTRACT

Objective: This study was designed to compare the antibacterial effect of nutmeg seed extracts (Myristica Fragrans Houtt) on the common causative bacteria of skin infection, Staphylococcus aureus and S. epidermidis. Methods: The antibacterial effect of the nutmeg seed extracts was analyzed using the agar diffusion method. The determination of extracts Minimum Inhibitory Concentration (MIC) was done using the macrodilution method. In addition to the antibacterial effect conclusion, the antibacterial effect of the extract was evaluated on both staphylococcus strains using the neomycin sulfate as the standard antibiotic. The comparative method was the agar diffusion method, followed by the inhibitory diameters data plotting to log concentration of the extract or neomycin sulfate, thus an inhibition curve was obtained. Results: The antibacterial activity of nutmeg seed extract on S. aureus was stronger then to S. epidermidis. This data was supported by comparing the MIC value of the extract on both strains. The MIC value of the extract against S. aureus (0.156

Keywords: Myristica Fragrans Houtt, nutmeg seed, Staphylococcus aureus, Staphylococcus epidermidis, MIC, comparative study.


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