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Abstract

PREVENTION, CONTROL, INHIBITION, AND THERAPY OF HCV GT 4A VIRUS INFECTION IN EGYPT

Fadel M. Ali, Hala M. Ahmed*, A. Elmaghraby and A. Amin

ABSTRACT

Objective: Electric Field (ELF-EMFs) has great potential for modern biotechnological and new medical applications. This paper shows the effect of Electric Field (ELF-EMFs) on HCV GT 4a, which could imply a durable change in Clinical chemistry parameters and virological testing. Methods: Hepatitis C virus (HCV) infection has acute and chronic forms with most of the morbidity through the development of chronic liver disease. For this purpose, HCVGT 4a activities in Huh-7 and HepG-2 cell line media were exposed to different frequencies of amplitude modulated (AM) waves in the frequency range (from 1 Hz to 10 Hz) for a period of 30 minutes /day along four successive days no measurable effect of the square amplitude modulated waves (QAMW) was noticed after the experiment in the Huh-7 and Hep G-2 cells. At day (1, 2, 3, 4, 5, and 6) of the (PBMCs), supernatant was collected for each group then the liver enzymes (ALT), Measurement of complete blood count, determination the virological testing, Cultures were daily examined using the inverted microscope (Hund-Germany) for detection of cytopathic effect (CPE). The results: The data highly significant growth inhibition occurred in HCVGT 4a injected with Huh-7 and Hep G-2 cell line after exposure to 5.2 Hz QAMW for 30 minutes/day along four successive days. The mean ALT level was slightly higher in patients infected with HCV GT 4a non exposed Electromagnetic waves (410 U/L) than those infected with HCV GT 4a exposed Electromagnetic waves (90 U/L). There was a decrease of mean ALT levels during the course of treatment in both groups. Hemoglobin levels between the two groups decreased slightly during treatment the follow-up period. Neutrophil counts between the two groups decreased slightly during treatment but increased again during the follow-up period. The median baseline serum HCV RNA load was 9.7x105 IU/ml in patients with HCV GT 4 exposed infection (p<0.001), and 2.0x105 IU/ml in patients with HCV GT 4a non exposed infection (p <0.02).The viral isolates showed a gradually elevated replication activity on Huh-7 and Hep G-2 cells, where the cytological changes (90% CPE) could be detected within 1-6 days post viral inoculation compared with the negative control. Conclusions: The results tend to show that Electric Field (ELF-EMFs) are able to inactivate viral growth and have probably no serious impact in HCVGT 4a. The results indicated that the HCVGT 4a has frequencies with the bioelectric signals generated during cellular division.

Keywords: HCVGT 4a, Electromagnetic field (ELF-EMFs), Huh-7 and Hep G-2 cells.


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