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Abstract

OPTIMIZATION OF PHYSICAL CONDITIONS FOR L-THREONINE PRODUCTION BY AN Α,€-DIAMINOPIMILIC ACID AND THIAMINEHCL DUAL AUXOTROPHIC MUTANT CORYNEBACTERIUM GLUTAMICUM X1870

*Subhadeep Ganguly

ABSTRACT

The production of L-threonine by α,€-diaminopimilic acid and thiamine-HCl dual auxotrophic mutant Corynebacterium glutamicum X1870 was studied by optimizing different physical conditions. Production was increased significantly from 10.7mg/ml to 15.1 mg/ml with pH, 6.0; temperature, 290C; fermentation time, 96h; volume of medium, 30 ml and inoculums size, 4%.

Keywords: L-threonine, ?,€-diaminopimilic acid, thiamine-HCl, dual auxotrophic, Corynebacterium glutamicum.


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