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Abstract

IN VITRO STUDY ON FLORAL PARTS OF POMEGRANATE PUNICA GRANATUM L. CV. ‘GANESH’

Priyanka Kadam*

ABSTRACT

In vitro studies were undertaken with a view to study the regeneration potentiality of different floral explants of field grown mature plants. The primary and secondary cultures of segments of petals, entire filaments and anthers induced embryogenic callus on Murashige and Skoog‘s media supplemented with individual and combined plant growth regulators such as NAA and TDZ. The callus obtained on media containing individual NAA was more in mass and friable. Such callus was used for callus maintenance and initiation of suspension cultures. Subculture studies of callus on semisolid nutrient media supplemented with natural additives like casein hydrolysate (25mg/l, 50mg/l,100mg/l) coconut water (10%,15% and 20%) and Abscisic acid (1mg/l,2mg/l, 3mg/l), revealed the presence of secondary embryogenesis on media. On the contrary, from the embryogenic callus induced on media containing combined PGRs, developed precociously germinated somatic embryos on media supplemented with activated charcoal (2%) and adenine sulphate (4mg/l) and coconut water (15%). The potentiality of embryogenic callus induction, precocious germination of somatic embryos, varied among the different explants depending upon the added growth regulator.

Keywords: NAA and TDZ.


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