WJPR Citation New

  All Since 2011
 Citation  2903  2393
 h-index  27  24
 i10-index  68  60

Login

Best Paper Awards

World Journal of Pharmaceutical Research (WJPR) will give best paper award in every issue in the form of money along with certificate to promote research activity of scholar.
            Best Paper Award :
Dr. Muhammad Baqir MR Fakhrildin
Download Article: Click Here

Search

Track Your Article

Abstract

AMELIORATIVE EFFECT OF PAPAIN ON BEHAVIORAL AND BIOCHEMICAL ALTERATIONS IN IN-SILICO AND IN-VIVO MODELS OF NEUROPATHIC PAIN IN RATS

Swapna Pokkula, Dr. Santh Rani Thaakur*, Vagolu Siva Krishna and Dharmarajan Sriram

ABSTRACT

The present study investigated the ameliorative effect of Papain (PN) on N-methyl D-aspartate (NMDA) receptor down-regulation pathway by using in-silico studies and on oxidative stress in-vivo model of neuropathic pain in rats via partial sciatic nerve ligation (PSNL). Insilico molecular docking study of Papain with NMDA receptors was carried out especially with active pocket of NR2B to know its binding affinity. In in-vivo studies, briefly the rats were randomly divided into sham, vehicle, Pregabalin (standard) and PN treated groups. PSNL was carried out in vehicle and PN treated rats but not in sham and PN (100) treatment control group. PN 50 and 100 mg/kg was administered for a period of 21 days after PSNL. Behavioral alterations were assessed by foot deformity score, cold-allodynia and motor co-ordination tests. On 21st day after behavioral assessment, the rats were sacrificed; sciatic nerve was collected for biochemical estimations of superoxide dismutase (SOD), catalase (CAT), lipid peroxide levels (MDA) and glutathione reductase (GR) levels. In-silico studies revealed that affinity of ligand PN with the NR2B (NMDA) receptor showed best antagonistic effects compared to standard pregabalin. From In-vivo studies, it was observed that PN significantly (p<0.001) attenuated PSNL induced in behavior and biochemical changes indicating its neuroprotection through antioxidant and antiglutaminergic mechanisms.

Keywords: Papain, In-silico docking studies, NR2B, In-vivo studies, Neuropathic pain, Oxidative stress.


[Full Text Article]

Call for Paper

World Journal of Pharmaceutical Research (WJPR)
Read More

Email & SMS Alert

World Journal of Pharmaceutical Research (WJPR)
Read More

Article Statistics

World Journal of Pharmaceutical Research (WJPR)
Read More

Online Submission

World Journal of Pharmaceutical Research (WJPR)
Read More