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Abstract

INVESTIGATION OF ANTI-BACTERIAL ACTIVITY OF CUPRESSUS TORULOSA LEAF EXTRACT

Ramesh Raj Padhaya, Suvechha Bhandari, Mribha Manandhar, Amrit Khanal, Sajan Maharjan*

ABSTRACT

The aim of present study was to investigate antibacterial activity of Cupressus torulosa leaf extract. The dried material was extracted by using 3 different solvents namely methanol, hexane and ethyl actetate. Plant extracts were subjected to phytochemical screening which showed the presence of Alkaloid, Glycoside, tannins, carbohydrate and amino acids. In test for antibacterial activity against four microorganisms (Streptococcus pyogens, Acinetobacter calcoaceticus, Escherechia coli, Staphylococcus aureus), methanol extracts showed highest antibacterial activity whereas hexane extracts showed less activity. This activity was assessed by measuring the diameter of ZOI of four concentrations used. ZOI was larger in 200ug/ml and least in 25ug/ml concentration of extracts. The largest zone of inhibition (15mm) was obtained with S.pyogens in methanol extract. When hexane extract was used, largest ZOI was 10mm against acinetobacter calcoaceticus. When ethyl acetate extracts was used, largest ZOI was 12mm against staphylococcus aureus. Among four pathogenic bacteria used all were found susceptible (ZOI<10) to plant extracts at different concentrations in methanol extracts. In ethyl acetate extracts, S.aureus and E. coli were found less susceptible whereas in hexane extracts, Acinetobacter calcoaceticus was found less susceptible.

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